Purpose Vascular reconstruction in infected fields poses a significant challenge in vascular surgery owing to the surgical complexity and high rates of re-infection, morbidity, and mortality. Mycotic aneurysms and Vascular and Endovascular Graft Infections (VEGI) are complex conditions requiring multidisciplinary management in high volume centres. Reconstruction often involves extra-anatomic bypasses, which are limited by feasibility and effectiveness of avoiding the infected fields, or in-situ reconstructions where conduit material often requires compromise. Biosynthetic conduits offer an off-the-shelf option for reconstruction that carries the structural benefit of prosthetic material while holding the anti-infective benefits and incorporation seen in biological reconstructions. Methodology This case series outlines three cases of successful in-situ reconstruction of aortoiliac infections using off-the-shelf Omniflow II grafts. Two patients were identified with VEGI and one with a mycotic aneurysm. All three patients had confirmed microbiological pathogens, and were treated with biosynthetic in-situ bypasses. Follow up of at least 2 years was reviewed, including surveillance imaging Results All three patients have demonstrated long-term graft patency, and freedom from re-infection or aneurysmal dilatation. Surveillance duplex ultrasound identified hemodynamically significant stenosis in two patients, though neither has required intervention. One patient has since required bilateral major limb amputations due to diabetic foot sepsis, unrelated to the original aortoiliac pathology. Conclusion Biosynthetic conduits represent a viable choice for in-situ reconstruction of aortoiliac systems post infection. This case series highlights the feasibility of this technology in a variety of vascular infections, as well as the potential long-term durability. However, more evidence across a broader patient cohort is required to incorporate this into best practice guidelines.